CDK4 antibody

Catalog No. ABIN112142

Product Details anti-CDK4 Antibody

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: This antibody reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle, it does not cross-react with cyclins. Especially suitable for immunoprecipitation, mab DCS-35 precipitates the cdk4/cyclin complex.

Purification: Protein A affinity chromatography

Immunogen: Human recombinant full-length cdk4 polypeptide

Clone: DCS-35

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation: 1: 3 - 1: 10, 1 - 4 h at RT or overnight at 2-8C. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water

Buffer: PBS buffer, pH 7.4 containing 0.09 % NaN3, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4 (CDK4 Products)

Background: Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3

Gene ID: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size