Cyclin-Dependent Kinase 4 (CDK4) antibody

Details for Product No. ABIN112142
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Antigen
Synonyms zgc:153726, CMM3, PSK-J3, Crk3, 8-6, CDK4, CDK4/6, CG5072, Cdk4/6, DmCdk4, Dmel\\CG5072, Pk53C, Pk?7, cdk, cdk4, cdk4/6, dCdk4, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, xcdk4
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(190), (64), (48), (18), (14), (4), (1)
Host
Mouse
(111), (87), (7), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (6), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunoprecipitation (IP), Western Blotting (WB)
(189), (67), (54), (42), (31), (18), (17), (12), (10), (10), (8), (5), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN112142
346.50 $
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Immunogen Human recombinant full-length cdk4 polypeptide
Clone DCS-35
Isotype IgG1
Specificity This antibody reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle, it does not cross-react with cyclins. Especially suitable for immunoprecipitation, mab DCS-35 precipitates the cdk4/cyclin complex.
Purification Protein A affinity chromatography
Alternative Name CDK4
Background Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3
Gene ID 1019
NCBI Accession NP_000066
UniProt P11802
Research Area Cell Cycle
Application Notes Immunoprecipitation: 1: 3 - 1: 10, 1 - 4 h at RT or overnight at 2-8C. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1 mL dist. water
Buffer PBS buffer, pH 7.4 containing 0.09 % NaN3, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Lukas, Jensen, Bartkova et al.: "Immunohistochemical analysis of the D-type cyclin-dependent kinases Cdk4 and Cdk6, using a series of monoclonal antibodies." in: Hybridoma, Vol. 18, Issue 3, pp. 225-34, 1999 (PubMed).

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