CDK4 antibody (C-Term)

Catalog No. ABIN1881714

Cited in 1 publication. The Rabbit Polyclonal anti-CDK4 antibody (ABIN1881714) specifically detects CDK4 in WB, IF and IHC (p). The antibody is reactive with Rat samples.

Quick Overview for CDK4 antibody (C-Term) (ABIN1881714)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDK4 Antibody

Binding Specificity: AA 272-303, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Rat Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-303 amino acids from the C-terminal region of rat Cdk4.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-CDK4 antibody (ABIN1881714)

Cho, Phillips, Khan, Weaver: "Cloning of the rat cyclin-dependent kinase 4 cDNA: implication in proliferation-dependent expression in rat tissues." in: Biochemical and biophysical research communications, Vol. 191, Issue 3, pp. 860-5, (1993) (PubMed).

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: Cdk4

Background: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Molecular Weight: 33799

NCBI Accession: NP_446045

UniProt: P35426

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size