CDK4 antibody (AA 201-303)

Catalog No. ABIN3043745

There are 10+ publications for this product available. The Rabbit Polyclonal anti-CDK4 antibody is suitable to detect CDK4 in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for CDK4 antibody (AA 201-303) (ABIN3043745)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDK4 Antibody

Binding Specificity: AA 201-303

Purpose: Anti-Cdk4 Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse,rat Cdk4.

Characteristics: Anti-Cdk4 Antibody. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Cdk4 recombinant protein (Position: G201-E303). Human Cdk4 shares 94.2% amino acid (aa) sequence identity with both mouse and rat Cdk4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat Immunofluorescence, 5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-CDK4 antibody (ABIN3043745)

Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Zhang, Zhao, Huang, Meng, Bian, Li: "Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e95520, (2014) (PubMed).

Wang, Huang, Han, Xue, Li, Liu, Shan, Wang, Li, Li: "The effects of artemisinin on the proliferation and apoptosis of vascular smooth muscle cells of rats." in: Cell biochemistry and function, Vol. 32, Issue 2, pp. 201-8, (2014) (PubMed).

Li, Xu, Chu, Gao, Wang, Nie, Yang, Lv: "Molecular mechanism of inhibitory effects of CD59 gene on atherosclerosis in ApoE (-/-) mice." in: Immunology letters, Vol. 156, Issue 1-2, pp. 68-81, (2013) (PubMed).

Cai, Xu, Zhang: "Bone marrow stromal cells induce cell cycle arrest in reactive astrocytes in vitro." in: Neuroscience letters, Vol. 522, Issue 1, pp. 62-6, (2012) (PubMed).

Li, Xu, Li, Zou, Saimaiti, Simayi, Wang, Zhang, Yuan, Zhang: "Stat6 activity-related Th2 cytokine profile and tumor growth advantage of human colorectal cancer cells in vitro and in vivo." in: Cellular signalling, Vol. 24, Issue 3, pp. 718-25, (2012) (PubMed).

Zhang, Ge, Sun, Cao, Wu, Guo, Wang: "Effect of bone morphogenetic protein-2 on proliferation and apoptosis of gastric cancer cells." in: International journal of medical sciences, Vol. 9, Issue 2, pp. 184-92, (2012) (PubMed).

Liu, Dai, Lu, Wei, Ha, Rong, Mu, You, Li, Guo: "LYG-202 inhibits the proliferation of human colorectal carcinoma HCT-116 cells through induction of G1/S cell cycle arrest and apoptosis via p53 and p21(WAF1/Cip1) expression." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 89, Issue 3, pp. 287-98, (2011) (PubMed).

Han, Liu: "EGR-1 decoy ODNs inhibit vascular smooth muscle cell proliferation and neointimal hyperplasia of balloon-injured arteries in rat." in: Life sciences, Vol. 86, Issue 7-8, pp. 234-43, (2010) (PubMed).

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background:

Background: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. And CDK4 expression and activity are required for cytokine responsiveness in T cells.

Gene Full Name: cyclin dependent kinase 4

Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Molecular Weight: 34 kDa

Gene ID: 1019

UniProt: P11802

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size