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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Monoclonal (RV202)
Host
Alternatives

Mouse
Reactivity
Alternatives

Dog (Canine), Chicken, Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
5 references available
Catalog no. ABIN112354
Quantity 0.1 mg  (1.0 mg/ml)
Price 495.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives back to top

Alternative name Vimentin
UniProt P08670
Immunogen Cytoskeletal vimentin extract of calf lens.
Cross-Reactivity Chicken, Mammalian
Isotype IgG1
Clone RV202
Description Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFPhowever often deviates from the tissue-specific and developmentally regulated pattern ofexpression. Besides its typical expression in most cultured cells, vimentin is alsoexpressed together with several other IFPs during early stages of development. Asdifferentiation proceeds, vimentin is exchanged for the tissue-specific intermediatefilament type. Also in cancers, vimentin is often expressed in addition to thetissue-specific IFP.

Application Details
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Application Notes RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS with 0.09% Sodium Azide as preservative.
Storage Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988 (PubMed).

Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Mouse (146), Rabbit (140), Chicken (17), Sheep (6), Guinea Pig (4), Rat (4), Goat (3)
Reactivities Human (264), Rat (Rattus) (112), Mouse (Murine) (102), Cow (Bovine) (63), Pig (Porcine) (56), Chicken (46), Dog (Canine) (37), Sheep (Ovine) (24), Horse (Equine) (18), Mammalian (12), Monkey (12), Goat (11), Hamster (9), Zebrafish (6), Guinea Pig (5), Amphibian (4), Xenopus laevis (3), Potoroo (2), Zebrafish (Danio rerio) (2), All Species (1), Cat (Feline) (1), Fish (1), Frog (1), Primate (1), Rabbit (1)
Applications Western Blotting (WB) (203), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (110), ELISA (109), Immunofluorescence (IF) (89), Immunocytochemistry (ICC) (76), Immunohistochemistry (IHC) (66), Flow Cytometry (FACS) (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (30), Immunoprecipitation (IP) (22), Immunohistochemistry (Fixed) (IHC (fx)) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectrophoresis (IEP) (3), Intracellular Staining (IS) (3), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (12), Biotin (11), Alexa Fluor 488 (7), PE (7), HRP (6), Dyomics 547 (4), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), DY547 (2), DyLight 549 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes pSer82 (20), pSer72 (13), N-Term (11), Internal Region (6), pSer56 (6), C-Term (4), pSer38,N-Term (3), pSer72,Internal Region (3), pTyr61,N-Term (3), Ser82 (2), AA 2-466 (1), AA 300-350 (1), AA 400-500 (1), AA 70-120 (1), Center (1), pSer38 (1), pSer83 (1), pTyr996 (1)