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Details for Product No. ABIN112354

Vimentin (VIM) antibody

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Antigen
Synonyms CTRCT30, cb28, VIM, Vimentin, vim, vime
Reactivity
»Alternatives Dog (Canine), Chicken, Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 5 references available
Catalog no. ABIN112354
Quantity 0.1 mg
Price
478.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Cytoskeletal vimentin extract of calf lens.
Clone RV202
Isotype IgG1
Specificity RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. Reacts with human, mouse, rat, hamster, chicken, goat, canine and monkey.
Purification Purified
Alternative Name Vimentin
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Alternate names: VIM
Gene ID 7431
NCBI Accession NP_003371.2
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Application Notes RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS with 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized antibody at 2-8°C for one month or at -20°C for longer. Aliquot and store the reconstituted antibody at -20°C.
Expiry Date 12 months
Background publications Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988 (PubMed).

Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts (203), (169), (20), (6), (4), (4), (4)
Reactivities (353), (131), (119), (67), (62), (39), (37), (13), (13), (12), (8), (8), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Applications (292), (141), (123), (96), (83), (71), (38), (35), (32), (30), (15), (6), (3), (3), (3), (2), (2), (2), (1), (1)
Conjugates (16), (14), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Epitopes (19), (16), (16), (14), (8), (8), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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