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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Vimentin (VIM) antibody
|Synonyms||FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime|
Dog (Canine), Chicken, Goat, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|5 references available|
|Quantity||0.1 mg (1.0 mg/ml)|
|Price||495.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 6 to 8 Business Days|
|Immunogen||Cytoskeletal vimentin extract of calf lens.|
|Description||Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFPhowever often deviates from the tissue-specific and developmentally regulated pattern ofexpression. Besides its typical expression in most cultured cells, vimentin is alsoexpressed together with several other IFPs during early stages of development. Asdifferentiation proceeds, vimentin is exchanged for the tissue-specific intermediatefilament type. Also in cancers, vimentin is often expressed in addition to thetissue-specific IFP.|
|Application Notes||RV202 is suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry onfrozen tissues and Flow cytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/1000 for Immunoblotting applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.|
|Buffer||PBS with 0.09% Sodium Azide as preservative.|
|Storage||Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Restrictions||For Research Use only|
Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).
Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).
Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).
Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988 (PubMed).
Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).