Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN94491
Antigen
Reactivity
Mammalian
811
308
300
122
107
72
62
54
52
39
37
35
19
15
11
10
8
5
5
3
2
2
2
2
1
1
1
1
1
1
Host
Mouse
413
372
28
12
11
8
7
3
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
35
22
21
14
11
11
11
11
11
11
11
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
642
329
229
195
182
177
90
53
49
48
41
20
15
14
13
9
8
3
3
3
2
1
1
1
1
1
1
Options
Immunogen Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
Clone VI-01
Isotype IgM
Specificity The antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.
Cross-Reactivity (Details) Mammalian
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP). Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin. Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Pathways Caspase Cascade in Apoptosis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Vimentin (VIM) antibody (ABIN94491) Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mo...
Image no. 2 for anti-Vimentin (VIM) antibody (ABIN94491) Immunofluorescence staining (rat basophils)
Image no. 3 for anti-Vimentin (VIM) antibody (ABIN94491) Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast...
Image no. 4 for anti-Vimentin (VIM) antibody (ABIN94491) Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detec...
Product cited in: Bacáková, Mares, Lisá, Svorcík: "Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions." in: Biomaterials, Vol. 21, Issue 11, pp. 1173-9, 2000 (PubMed).

Dráberová, Dráber, Havlícek, Viklický: "A common antigenic determinant of vimentin and desmin defined by monoclonal antibody." in: Folia biologica, Vol. 32, Issue 5, pp. 295-303, 1989 (PubMed).