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Vimentin antibody (AA 2-466)

Name: Vimentin antibody (AA 2-466)
Brand: antibodies-online
SKU: ABIN7193582
Price: 511.5 USD
Availability: InStock

VIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

Western blot analysis using VIM mAb against HEK293 (1) and VIM (AA: 2-466)-hIgGFc transfected HEK293 (2) cell lysate. (Vimentin antibody (AA 2-466))

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using VIM mouse mAb with DAB staining. (Vimentin antibody (AA 2-466))

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (Vimentin antibody (AA 2-466))

ELISA

Western blot analysis using VIM mAb against human VIM (AA: 2-466) recombinant protein. (Vimentin antibody (AA 2-466))

Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264. (Vimentin antibody (AA 2-466))

Flow cytometric analysis of Hela cells using VIM mouse mAb (green) and negative control (red). (Vimentin antibody (AA 2-466))

FACS

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIM mouse mAb with DAB staining. (Vimentin antibody (AA 2-466))

IHC

7 images

Catalog No. ABIN7193582

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Binding Specificity
32

18

16

16

15

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466
Reactivity
332

125

124

61

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
190

128

9

6

4

4

2
Mouse
Compatible Secondaries
Clonality
198

144
Monoclonal
Conjugate
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
281

131

99

97

95

65

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Purification

purified

Immunogen

Purified recombinant fragment of human VIM (AA: 2-466) expressed in E. coli.

Clone

3A1F2

Isotype

IgG2a

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KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

WB:1:500 - 1:2000, IHC:1:200 - 1:1000, FCM:1:200 - 1:400, ELISA:1:10000,

Restrictions

For Research Use only
Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Target

Vimentin (VIM)

Alternative Name

VIM (VIM Products)

Synonyms

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Molecular Weight

53.7 kDa

Gene ID

7431

Pathways

Caspase Cascade in Apoptosis