Keratin 7 (KRT7) antibody

Details for Product No. ABIN114453
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Antigen
Synonyms CK7, K2C7, K7, SCL, D15Wsu77e, Krt2-7, KRT7
Reactivity
Human
(185), (46), (42), (8), (7), (7), (4), (4), (4), (3), (1), (1), (1)
Host
Mouse
(108), (100), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (7), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(146), (91), (56), (49), (49), (39), (33), (30), (20), (10), (10), (3), (2), (1)
Pubmed 7 references available
Quantity 0.1 mg
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Catalog No. ABIN114453
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Immunogen A cytoskeletal preparation of OTN- 1 ovarian carcinoma cell line
Clone OVTL-12-30
Isotype IgG1
Specificity This antibody reacts exlcusively with Cytokeratin 7 which is present in specific glandular- type epithelia and most carcinomas derived thereof. Species Reactivity: Tested: Human.
Purification Purified
Alternative Name Cytokeratin 7
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.
Alternate names: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin
Gene ID 3855
NCBI Accession NP_005547
UniProt P08729
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Immunohistochemistry on frozen and paraffin embedded sections (1: 100 - 1: 200 with anABC method). Antibody ABIN114453 is effective on frozen tissue after fixation in methanol. It is particularly effective on paraffin-embedded tissue, for which pretreatment with e. g.0.1% pronase / 0.1% trypsine in PBS or 0.5% pepsine in 0.01N HCl or microwavepretreatment is required. Also suitable for immunocytochemistry, immunoblotting (1: 100 - 1: 1000) and flowcytometry (1: 100 - 1: 200). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS containing 0.09% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the product undiluted Prior to reconstitution at 2-8°C and after reconstitution (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Crooks: "The pathological concept of diffuse axonal injury; its pathogenesis and the assessment of severity." in: The Journal of pathology, Vol. 165, Issue 1, pp. 5-10, 1991 (PubMed).

van Niekerk, Boerman, Ramaekers et al.: "Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas." in: The American journal of pathology, Vol. 138, Issue 2, pp. 455-63, 1991 (PubMed).

van de Molengraft, van Niekerk, Jap et al.: "OV-TL 12/30 (keratin 7 antibody) is a marker of glandular differentiation in lung cancer." in: Histopathology, Vol. 22, Issue 1, pp. 35-8, 1993 (PubMed).

Nanashima, Tagawa, Yasutake et al.: "Deletion of chromosome 11 and development of colorectal carcinoma." in: Cancer detection and prevention, Vol. 21, Issue 1, pp. 7-11, 1997 (PubMed).

Torenbeek, Lagendijk, Van Diest et al.: "Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma." in: Histopathology, Vol. 32, Issue 1, pp. 20-7, 1998 (PubMed).

General Smedts, Ramaekers, Link et al.: "Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology." in: Virchows Archiv : an international journal of pathology, Vol. 425, Issue 2, pp. 145-55, 1994 (PubMed).

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