Cytokeratin 7 antibody

Catalog No. ABIN114453

Product Details anti-Cytokeratin 7 Antibody

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts exlcusively with Cytokeratin 7 which is present in specific glandular-type epithelia and most carcinomas derived thereof.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified

Immunogen: A cytoskeletal preparation of OTN- 1 ovarian carcinoma cell line

Clone: OVTL-12-30

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin embedded sections (1: 100 - 1: 200 with anABC method). Antibody ABIN114453 is effective on frozen tissue after fixation in methanol. It is particularly effective on paraffin-embedded tissue, for which pretreatment with e. g.0.1 % pronase / 0.1 % trypsine in PBS or 0.5 % pepsine in 0.01N HCl or microwavepretreatment is required. Also suitable for immunocytochemistry, immunoblotting (1: 100 - 1: 1000) and flowcytometry (1: 100 - 1: 200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the product undiluted Prior to reconstitution at 2-8 °C and after reconstitution (in aliquots) at -20 °C.

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7 (KRT7 Products)

Synonyms: CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

Gene ID: 3855

NCBI Accession: NP_005547

UniProt: P08729