Recombinant Cytokeratin 7 antibody

Catalog No. ABIN6939879

This Rabbit Monoclonal antibody specifically detects Cytokeratin 7 in WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Cytokeratin 7 antibody (ABIN6939879)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: KRT7-1499R

Product Details anti-Cytokeratin 7 Antibody

Purpose: Recombinant Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Specificity: It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Purification: 200ug/ml of Ab Purified by Protein A Column.

Immunogen: Recombinant full-length human KRT7 protein

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: HeLa cells. Carcinoma of ovary, lung, cervix or breast.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: KRT7

Background:

CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

cervix or breast., HeLa cells. Carcinoma of ovary, Lung

Molecular Weight: 55kDa

Gene ID: 3855, 411501

UniProt: P08729