Cytokeratin 7 antibody

Catalog No. ABIN6939874

The Mouse Monoclonal anti-Cytokeratin 7 antibody is suitable to detect Cytokeratin 7 in samples from Human. It has been validated for IHC, IF and FACS.

Quick Overview for Cytokeratin 7 antibody (ABIN6939874)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: KRT7-903

Product Details anti-Cytokeratin 7 Antibody

Purpose: Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Specificity: Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

No Cross-Reactivity: Ferret, Mouse (Murine), Rabbit, Rat (Rattus)

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human KRT7 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: A431, HeLa cells, Breast cancer.

Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris Buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: KRT7

Background:

CK7, K2C7, K7, Keratin 55k Type II Cytoskeletal, Keratin 7, Keratin Simple Epithelial Type 1 k7, Keratin Type II Cytoskeletal 7, Keratin, 55K Type II Cytoskeletal, Krt2-7, KRT7, Sarcolectin, SCL, Type ii Mesothelial Keratin k7, Type-II Keratin Kb7

A431, Breast cancer., HeLa cells

Molecular Weight: 54kDa

Gene ID: 3855, 411501

UniProt: P08729