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Details for Product No. ABIN115263

Breast Cancer 1 (BRCA1) (C-Term), (AA 1839-1863) antibody

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Synonyms BRCA1
»Alternatives C-Term, AA 1839-1863
»Alternatives Human
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 5 references available
Catalog no. ABIN115263
Quantity 1 mL
995.50 $   Plus shipping costs $45.00
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Immunogen Peptide corresponding to amino acids 1839-1863 of the c-terminus of BRCA1
Clone GLK-2
Isotype IgM
Specificity BRCA1 recognizes a 22 kD protein. This antibody stains 94 % of ovarian carcinomas with a mutation in exon 11. However, staining was absent in 100 % of tumors with mutations other than exon 11. Cellular Localization: Cytoplasmic.
Purification None
Alternative Name BRCA1 / RNF53
Background BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full length natures identified.
Alternate names: Breast cancer type 1 susceptibility protein, RING finger protein 53
Gene ID 672
NCBI Accession NP_009225.1
UniProt P38398
Application Notes Immunohistochemistry on frozen and paraffin sections (ABC method: 1/15 - 1/50, Formalinfixed paraffin embedded tissue sections require high temperature antigen unmasking with10 mM citrate buffer, pH 6.0 prior to immunostaining, Incubation period of 30-60 minutesat room temperature). Use ovarian carcinoma as positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer Tissue culture supernatant with 0.05 % sodium azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Kashima, Oite, Aoki et al.: "Screening of BRCA1 mutation using immunohistochemical staining with C-terminal and N-terminal antibodies in familial ovarian cancers." in: Japanese journal of cancer research : Gann, Vol. 91, Issue 4, pp. 399-409, 2000 (PubMed).

Bur, Zimarowski, Schnitt et al.: "Estrogen receptor immunohistochemistry in carcinoma in situ of the breast." in: Cancer, Vol. 69, Issue 5, pp. 1174-81, 1992 (PubMed).

Graham, Jin, Lloyd: "Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization." in: The American journal of surgical pathology, Vol. 15, Issue 5, pp. 475-85, 1991 (PubMed).

Pertschuk, Kim, Nayer et al.: "Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Histopathologic, demographic, and biochemical correlations and relationship to endocrine response and survival." in: Cancer, Vol. 66, Issue 8, pp. 1663-70, 1990 (PubMed).

Kumar, Green, Stack et al.: "Functional domains of the human estrogen receptor." in: Cell, Vol. 51, Issue 6, pp. 941-51, 1988 (PubMed).

Alternatives for antigen "Breast Cancer 1 (BRCA1)", type "Antibodies"
Hosts (295), (80), (4), (1)
Reactivities (357), (35), (24), (13), (2), (2), (1), (1), (1), (1)
Applications (218), (96), (90), (84), (72), (70), (63), (3), (2), (2), (1), (1), (1)
Conjugates (16), (16), (10), (9), (9), (9), (9), (9), (9), (9), (9), (6), (3), (3), (1)
Epitopes (37), (33), (24), (17), (15), (15), (15), (14), (13), (12), (12), (7), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)