MSTN antibody

Catalog No. ABIN115510

This Chicken Polyclonal antibody specifically detects MSTN in EIA. It exhibits reactivity toward Human.

Quick Overview for MSTN antibody (ABIN115510)

Target: MSTN (Myostatin (MSTN))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This MSTN antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-MSTN Antibody

Sequence: Total 248 AA. MW: 28 kDa (calculated ). 243 AA of recombinan t human Myostatin Propeptide and 5 extra AA: MGNENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN Remarks: The amino acid sequence of the recombinan t Human Myostatin Propeptide is 100% homologous with the amino acid sequence of the Human Myostatin Propeptide without signaling sequence.

Specificity: The recombinant human myostatin propeptide is 100 % homologous with the human serum myostatin propeptide.

Purification: Immunoaffinity chromatography.

Immunogen: Recombinant human myostatin propeptide from E. coli.

Application Details

Application Notes: Suitable for ELISA: Titer is defined by indirect ELISA, it is: > 1/100,000 for antibodyconcentration 1 mg/mL, 25 ng of antigen are coated per well, and is then defined at a pointof maximal decrease of the titration curve.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Concentration: 1.0 mg/mL

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2 containing no preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20° C or below.

Target Details for MSTN

Target: MSTN (Myostatin (MSTN))

Alternative Name: Myostatin

Background: Myostatin (GDF-8), a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70 % ) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.Synonyms: GDF-8, GDF8, Growth differentiation factor 8, MSTN

Gene ID: 2660

NCBI Accession: NP_005250

UniProt: O14793