Retinoblastoma-Like 2 (p130) (RBL2) (C-Term) antibody

Details for Product No. ABIN116943
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Antigen
Synonyms RBL2, RBR2, RRB2a, PRB2, RBR-2, Rb2, p130, P130
Epitope
C-Term
(15), (9), (8), (5), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(98), (47), (47), (37), (37), (23), (3), (2), (2), (2)
Host
Rabbit
(77), (24)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(56), (36), (29), (25), (23), (11), (7), (7), (5), (2)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN116943
390.50 $
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Immunogen Rb2 (p130) peptide corresponding to the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH). Genename: RBL2
Specificity Antiserum will specifically react with a 130 kDa Rb2 protein from Human, Rat and Mouse tissue. No reaction was observed against other related tumor supressor proteins. Cross reactivity with Rb2 (p130) from other species may also occur.
Purification Delipidation and Defibrination.
Alternative Name RBL2 / p130
Background P130/pRb2, p107 and Rb together make up the Rb family of cell cycle regulatory proteins. As a family of proteins, the different members share several structural and functional features which include two distinct "pocket domains", interaction with viral gene products, interaction with members of the E2F family of proteins and changes in phosphorylation states leading to modulation of activity and cell cycle expression. The p130 protein is ubiquitously expressed with highest levels in terminally differentiated cells where it presumably functions to maintain the cells in a G0 state and as such has been used as a marker for G0. Depending on position within the cell cycle, p130 can exist in 4 different forms as a function of phosphorylation with Form 0 being the unphosphorylated form and the other three forms being referred to as Forms 1-3 correlating with increasing degrees of phosphorylation. Changes in levels of p130 expression have been reported in several cancers including endometrial, small cell lung, and neuroblastoma.
Alternate names: 130 kDa retinoblastoma-associated protein, RB2, RBR-2, Retinoblastoma-like protein 2, Retinoblastoma-related protein 2, p130, pRb2
Gene ID 5934
NCBI Accession NP_005602
UniProt Q08999
Application Notes Suitable for ELISA, Immunoprecipitation, Immunoblotting and other immunologicalmethods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/500-1/20,000. Western Blot: 1/500-1/2,000. Immunoprecipitation: 1/100. Immunohistochemistry on Paraffin Embedded Tissue: 1/200-1/1,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Retinoblastoma-Like 2 (p130) (RBL2) (C-Term) antibody Immunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum. The staining shows the location of pRb2/p130 in developing mouse tissue. Other detection systems should yield similar results. Sections were cut at 5-7 um, mounted on glass and dried overnight at 37°C. All sections were deparaffinized in Xylene, rehydrated through a graded alcohol series and washed in phosphatebuffered saline (PBS). PBS was used for all subsequent washes and for antiserum dilution. Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum. Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min. Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20°C. Diaminobenzidine was used as the final chromogen. Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.
Background publications Claudio, Howard, Baldi et al.: "p130/pRb2 has growth suppressive properties similar to yet distinctive from those of retinoblastoma family members pRb and p107." in: Cancer research, Vol. 54, Issue 21, pp. 5556-60, 1994 (PubMed).

Yeung, Bell, Testa et al.: "The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19." in: Oncogene, Vol. 8, Issue 12, pp. 3465-8, 1993 (PubMed).

Mayol, Graña, Baldi et al.: "Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain." in: Oncogene, Vol. 8, Issue 9, pp. 2561-6, 1993 (PubMed).

Pucci, Claudio, Masciullo et al.: "pRb2/p130 promotes radiation-induced cell death in the glioblastoma cell line HJC12 by p73 upregulation and Bcl-2 downregulation." in: Oncogene, Vol. 21, Issue 38, pp. 5897-905, 2002 (PubMed).

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