Rub1 antibody

Details for Product No. ABIN116956, Supplier: Log in to see
Enzyme Immunoassay (EIA), Western Blotting (WB)
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Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Specificity This purified polyclonal antibody reacts with yeast Rub1. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Purification Multi-step process.
Background Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog Nedd8 are activated by the E1 ubiquitin-activating enzyme UBA2, that forms isopeptide linkages between thio esters. Nedd8 shows 80 % homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1-/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.Synonyms: NEDD8-like protein RUB1, Ubiquitin-like protein RUB1, YDR139C
Gene ID 851717
NCBI Accession NP_010423
UniProt Q03919
Application Notes Suitable for Immunoblotting (Western Blot: 1/500-1/2,000) and ELISA (1/1,000-1/5,000). Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation. A 6 kDa band corresponding to yeast Rub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.5 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Supplier Images
 image for anti-Rub1 antibody (ABIN116956) Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody, generated by immunization with...
Background publications Laufs, Nigrovic, Schneider, Oettgen, Del, Moskowitz, Blume, Perez-Atayde: "Giant cell myocarditis in a 12-year-old girl with common variable immunodeficiency." in: Mayo Clinic proceedings. Mayo Clinic, Vol. 77, Issue 1, pp. 92-6, 2002 (PubMed).

del Pozo, Dharmasiri, Hellmann, Walker, Gray, Estelle: "AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response." in: The Plant cell, Vol. 14, Issue 2, pp. 421-33, 2002 (PubMed).

Wang, Kang, Feng, Serino, Schwechheimer, Wei: "CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome." in: Molecular biology of the cell, Vol. 13, Issue 2, pp. 646-55, 2002 (PubMed).

del Pozo, Estelle: "The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 26, pp. 15342-7, 2000 (PubMed).

Kamura, Conrad, Yan, Conaway, Conaway: "The Rbx1 subunit of SCF and VHL E3 ubiquitin ligase activates Rub1 modification of cullins Cdc53 and Cul2." in: Genes & development, Vol. 13, Issue 22, pp. 2928-33, 2000 (PubMed).

Liakopoulos, Büsgen, Brychzy, Jentsch, Pause: "Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 10, pp. 5510-5, 1999 (PubMed).

Pozo, Timpte, Tan, Callis, Estelle: "The ubiquitin-related protein RUB1 and auxin response in Arabidopsis." in: Science (New York, N.Y.), Vol. 280, Issue 5370, pp. 1760-3, 1998 (PubMed).

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