NEDD8 antibody (AA 1-32)

Catalog No. ABIN3031967

Product Details anti-NEDD8 Antibody

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Binding Specificity: AA 1-32

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Rabbit

Purification: Purified

Immunogen: A portion of amino acids 1-32 from the human protein was used as the immunogen for this NEDD8 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the NEDD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the NEDD8 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: NEDD8 (NEDD8 Products)

Synonyms: NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody

Background: NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

UniProt: Q15843

Pathways: Ubiquitin Proteasome Pathway