Avidin antibody (FITC)

Catalog No. ABIN116962

Product Details anti-Avidin Antibody (FITC)

Target: Avidin (AVD)

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Avidin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Avidin.

Characteristics: Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 3.0 moles DTAF per mole of Rabbit IgG

Purification: Immunoaffinity chromatography

Immunogen: Avidin [Hen Egg White].

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 10.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Avidin

Target: Avidin (AVD)

Alternative Name: Avidin (AVD Products)

Background: The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD

Gene ID: 396260

NCBI Accession: NP_990651

UniProt: P02701