CD3 antibody

Catalog No. ABIN1177370

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS and Func. It is suitable to detect CD3 in samples from Human.

Quick Overview for CD3 antibody (ABIN1177370)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: HIT3a

Product Details anti-CD3 Antibody

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: Reacts with the human epsilon-chain, a 20 kDa subunit of CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show this antibody to be mitogenic when used in conjuction with pokeweed mitogen. CD3 plays a role in signal transduction during antigen recognition. HIT3a antibody does not stain intracellular CD3 unlike the other CD3 clone, UCHT1.

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway