CDC20 antibody (C-Term)

Catalog No. ABIN117946

This Rabbit Polyclonal antibody specifically detects CDC20 in WB, IP and EIA. It exhibits reactivity toward Human.

Quick Overview for CDC20 antibody (C-Term) (ABIN117946)

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC20 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-CDC20 Antibody

Binding Specificity: AA 486-499, C-Term

Sequence: Human CDC20 (fizzy) (499 aa 54.7 kDa): 1 maqfafesdl hsllqldapi pnapparwqr kakeaagpap spmraanrsh sagrtpgrtp 61 gkssskvqtt pskpggdryi phrsaaqmev asfllskenq sensqtptkk ehqkawalnl 21 ngfdveeaki lrlsgkpqna pegyqnrlkv lysqkatpgs srktcryips lpdrildape 181 irndyylnlv dwssgnvlav aldnsvylws assgdilqll qmeqpgeyis svawikegny 241 lavgtssaev qlwdvqqqkr lrnmtshsar vgslswnsyi lssgsrsghi hhhdvrvaeh 301 hvatlsghsq evcglrwapd grhlasggnd nlvnvwpsap geggwvplqt ftqhqgavka 361 vawcpwqsnv latgggtsdr hiriwnvcsg aclsavdahs qvcsilwsph ykelisghgf 421 aqnqlviwky ptmakvaelk ghtsrvlslt mspdgatvas aaadetlrlw rcfeldparr 481 rerekasaak sslihqgir

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.

Purification: Delipidation and defibrination.

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.

Application Details

Application Notes: Western blot: This antibody reacts with human CDC20 (fizzy) (1: 500 - 1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa bandcorresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressingCDC20 can be used as a positive control.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for CDC20

Target: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Alternative Name: CDC20

Background: CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.Synonyms: Cell division cycle protein 20 homolog, p55CDC

Gene ID: 991

NCBI Accession: NP_001246

UniProt: Q12834