Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1) (C-Term), (AA 94-108) antibody

Details for Product No. ABIN117957
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Synonyms 1500002P15Rik, AA517855, ROC1, hop21, BA554C12.1, RNF75
C-Term, AA 94-108
(17), (2), (2), (2), (2), (1), (1), (1), (1)
Caenorhabditis elegans (C. elegans), Human, Mouse (Murine), Zebrafish (Danio rerio)
(54), (23), (17), (2), (2)
(52), (3)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(40), (18), (12), (11), (10), (6), (5), (5), (2), (2), (1), (1)
Pubmed 11 references available
Quantity 0.1 mL
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Catalog No. ABIN117957
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Immunogen Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.
Sequence Human ROC1 (108 aa 12.2 kDa) : 1 maaamdvdtp sgtnsgagkk rfevkkwnav alwawdivvd ncaicrnhim dlciecqanq 61 asatseectv awgvcnhafh fhcisrwlkt rqvcpldnre wefqkygh
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.
Purification Delipidation and defibrination.
Alternative Name RBX1 / RNF75
Background ROC1 also known as RING-box protein 1, Rbx1, Regulator of cullins 1, RING finger protein 75, and ZYP protein, is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.Synonyms: E3 ubiquitin-protein ligase RBX1, Protein ZYP, RING finger protein 75, RING-box protein 1, ROC1, Regulator of cullins 1
Gene ID 9978
NCBI Accession NP_055063
UniProt P62877
Application Notes This antibody reacts with human ROC1 by Western blot (1: 500-1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation ofubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 isdetected. Most cell lines expressing ROC1 can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85 mg/mL (by Refractometry)
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C.
Supplier Images
anti-Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1) (C-Term), (AA 94-108) antibody Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto- tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Wertz, ORourke, Zhang et al.: "Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase." in: Science (New York, N.Y.), Vol. 303, Issue 5662, pp. 1371-4, 2004 (PubMed).

Yu, Yu, Liu et al.: "Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex." in: Science (New York, N.Y.), Vol. 302, Issue 5647, pp. 1056-60, 2003 (PubMed).

Zheng, Schulman, Song et al.: "Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex." in: Nature, Vol. 416, Issue 6882, pp. 703-9, 2002 (PubMed).

Furukawa, Zhang, McCarville et al.: "The CUL1 C-terminal sequence and ROC1 are required for efficient nuclear accumulation, NEDD8 modification, and ubiquitin ligase activity of CUL1." in: Molecular and cellular biology, Vol. 20, Issue 21, pp. 8185-97, 2000 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Périn J-P, Seddiqi, Charbonnier et al.: "Genomic organization and expression of the ubiquitin-proteasome complex-associated protein Rbx1/ROC1/Hrt1." in: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 45, Issue 8, pp. 1131-7, 2000 (PubMed).

Seol, Feldman, Zachariae et al.: "Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34." in: Genes & development, Vol. 13, Issue 12, pp. 1614-26, 1999 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Tan, Fuchs, Chen et al.: "Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha." in: Molecular cell, Vol. 3, Issue 4, pp. 527-33, 1999 (PubMed).

Skowyra, Koepp, Kamura et al.: "Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 662-5, 1999 (PubMed).

Kamura, Koepp, Conrad et al.: "Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase." in: Science (New York, N.Y.), Vol. 284, Issue 5414, pp. 657-61, 1999 (PubMed).

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