RBX1 antibody (Middle Region)

Catalog No. ABIN2792171

Product Details anti-RBX1 Antibody

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NQASATSEEC TVAWGVCNHA FHFHCISRWL KTRQVCPLDN REWEFQKYGH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RBX1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RBX1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 108 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RBX1 antibody (ABIN2792171)

Maerki, Olma, Staubli, Steigemann, Gerlich, Quadroni, Sumara, Peter: "The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis." in: The Journal of cell biology, Vol. 187, Issue 6, pp. 791-800, (2009) (PubMed).

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1 (RBX1 Products)

Synonyms: BA554C12.1 antibody, RNF75 antibody, ROC1 antibody, 1500002P15Rik antibody, AA517855 antibody, im:7137515 antibody, zgc:136444 antibody, RBX1 antibody, ATRBX1 antibody, F7C8.160 antibody, F7C8_160 antibody, HRT1 antibody, REGULATOR OF CULLINS-1 antibody, RING-BOX 1 antibody, RING-box 1 antibody, hop21 antibody, BEST:CK01110 antibody, CG16982 antibody, CK01110 antibody, Dmel\CG16982 antibody, EG:115C2.11 antibody, Rbx1 antibody, Rbx1/Roc antibody, Roc antibody, Roc1alpha antibody, anon-EST:Posey65 antibody, dRbx1 antibody, dRoc1 antibody, dRoc1a antibody, ring-box 1 antibody, ring-box 1, E3 ubiquitin protein ligase antibody, RING-box 1 antibody, RING-box protein 1 antibody, RING-box protein 1a antibody, Regulator of cullins 1a antibody, RBX1 antibody, Rbx1 antibody, rbx1 antibody, MGG_08844 antibody, LOC9308017 antibody, rbx-1 antibody, Roc1a antibody

Background: ROC1 encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.
Alias Symbols: ROC1, RNF75, BA554C12.1
Protein Interaction Partner: CDT1, CDC34, KEAP1, UBE2L6, UBE2M, CUL1, CUL3, CUL4B, UBE2D3, UBE2D2, UBE2D1, DAB2IP, UBC, TP53, PRAME, DCUN1D1, CUL2, DCUN1D3, MAP4K1, VHL, NEDD4, MTSS1, VRK2, HIST1H4A, GFPT1, CTNNB1, CCNE1, DCUN1D5, FBXL5, RHOBTB3, SAMHD1, UBC12, FBXL15, UBE2R2, SERTAD
Protein Size: 108

Molecular Weight: 12 kDa

Gene ID: 9978

NCBI Accession: NM_014248, NP_055063

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome