Tumor Necrosis Factor (TNF) antibody

Antigen: Tumor Necrosis Factor (TNF)

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN118027

Additional Information

Alternative name: Tumor necrosis factor (TNF-alpha)

UniProt: P06804

Immunogen: This antibody was produced from whole rabbit serum prepared by repeated immunizationswith recombinant mouse TNFα produced in E. coli.

Cross-Reactivity: Mouse (Murine)

Description: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharidestimulated macrophages, and causes tumor necrosis when injected into tumour bearingmice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated withendotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linkedunits, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta(lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysisor cytostasis of certain transformed cells, being synergistic with interferon gamma in itscytotoxicity. Although it has little effect on many cultured normal human cells, TNF alphaappears to be directly toxic to vascular endothelial cells. Other actions of TNF alphainclude stimulating growth of human fibroblasts and other cell lines, activatingpolymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1,prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated intreatment of certain cancers and AIDS Related Complex.

Characteristics: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Application Details

Application Notes: Western Blot: Use HRP Goat-anti-Rabbit IgG [H&L] (code R1364HRP) and TMB as asubstrate. A working dilution range of 1: 500 to 1: 2,000 is suggested for this application to detect TNFαfrom supernatants or lysates of 2 x 10e6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα.

Concentration: 1.1 mg/ml (by UV absorbance at 280 nm)

Purification: Purified

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing0.01% (w/v) Sodium Azide as preservative.

Storage: Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at-20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology

Restrictions: For Research Use only

Publications

Nedwin, Naylor, Sakaguchi et al.: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985 (PubMed).

Birkas Kovats, Palik, Faludi et al.: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006 (PubMed).