Tumor Necrosis Factor (TNF) antibody

Details for Product No. ABIN118027
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Antigen
Synonyms TNF, tnf, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, TNF-alpha, DIF, TNFA, TNFSF2, TNFalpha, Tnfa, Tnfsf1a, RATTNF, TNFa, cTNF, TNF-ALPHA, Cachectin, Tnf-alpha, tnfa-like
Reactivity
Mouse (Murine)
(492), (198), (98), (66), (64), (34), (23), (22), (10), (10), (8), (5), (5), (4), (4), (4), (2), (2), (1), (1)
Host
Rabbit
(328), (298), (97), (25), (12), (9), (4), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(85), (49), (24), (11), (8), (6), (6), (6), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(449), (389), (203), (111), (92), (86), (67), (60), (56), (52), (39), (30), (29), (20), (19), (5), (5), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 1 mg
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Catalog No. ABIN118027
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Immunogen Recombinant Mouse TNF-alpha produced in E.coli.
Specificity The antibody is directed against mature 17,000 MW Mouse TNF-alpha. It does not recognize Mouse TNF-beta (lymphotoxin). This antibody will recognize the cell- bound precursor of TNF-alpha as a 26,000 protein in immunoblots, particularly in denatured samples. Also useful for Neutralization of Mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. Species Reactivity: Tested: Mouse.
Purification Selective salt precipitation
Alternative Name Tumor necrosis factor (TNF-alpha)
Background Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Alternate names: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
Gene ID 10090
UniProt P06804
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Cancer, Virology
Application Notes Western Blot: Use HRP Goat-anti-Rabbit IgG [H&L] (code R1364HRP) and TMB as asubstrate. A working dilution range of 1: 500 to 1: 2,000 is suggested for this application to detect TNFαfrom supernatants or lysates of 2 x 10e6 endotoxin-stimulated mouse peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) mouse serumplus 10 ng/mL E. coli LPS. Immunohistochemistry: A dilution range of 1: 400 is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry using adilution of 1: 200 for staining of TNFα.
Restrictions For Research Use only
Format Liquid
Concentration 1.1 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 0.01% (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 2-8°C.
Expiry Date 12 months
General Nedwin, Naylor, Sakaguchi et al.: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985 (PubMed).

Birkas Kovats, Palik, Faludi et al.: "[Possible connection between ghrelin, resistin and TNF-alpha levels and the metabolic syndrome caused by atypical antipsychotics]" in: Neuropsychopharmacologia Hungarica : a Magyar Pszichofarmakológiai Egyesület lapja = official journal of the Hungarian Association of Psychopharmacology, Vol. 7, Issue 3, pp. 132-9, 2006 (PubMed).

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