CD3 antibody (APC)

Catalog No. ABIN118100

The Rat Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Mouse.

Quick Overview for CD3 antibody (APC) (ABIN118100)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: KT3

Product Details anti-CD3 Antibody (APC)

Specificity: This antibody recognises the CD3 antigen which is expressed by mature T cells.

Purification: Affinity chromatography on Protein A

Immunogen: Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: For optimal staining incubations should be performed at roomtemperature, The Fc region of monoclonal antibodies may bind non-specifically to cellsexpressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: Use of Sodium Szide as a preservative will substantially inhibit the enzyme activity of Horseradish Peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway