Integrin alpha M (ITGAM) antibody

Antigen: Integrin alpha M (ITGAM)

Synonyms: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Clonality: Monoclonal (M1-70)

Host: Rat

Reactivity: Human, Mouse (Murine), Rabbit

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN118145

Additional Information

Alternative name: CD11b / ITGAM

Swiss-Prot: P05555

Immunogen: T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line

Isotype: IgG2b  (Matching secondary antibodies)

Clone: M1-70

Description: CD11b is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Alternate Names: Integrin Alpha M Chain, MAC-1.

Specificity: This antibody recognises the CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1).

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Flow cytometry: 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Clone M1/70.15 has been reported to block iC3b binding to its receptor. ABIN118139 is recommended for this purpose.

Concentration: 1.0 mg IgG/ml

Buffer: This antibody is supplied as liquid, purified immunoglobulin fraction in PBS buffer with 0.09% sodium azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Springer, Galfre, Secher et al.: "Mac-1: a macrophage differentiation antigen identified by monoclonal antibody." in: European journal of immunology, Vol. 9, Issue 4, pp. 301-6, 1979 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Beller, Springer, Schreiber: "Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor." in: The Journal of experimental medicine, Vol. 156, Issue 4, pp. 1000-9, 1983 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).