CD11b antibody (Integrin alpha M) (FITC)

Details for Product anti-ITGAM Antibody No. ABIN93959
Antigen
Reactivity
Human
613
402
134
77
32
30
29
22
15
13
10
10
8
6
5
5
4
4
2
Host
Mouse
453
333
157
7
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD11b antibody is conjugated to FITC
133
111
83
54
12
12
11
11
11
10
10
10
9
9
6
5
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
748
250
205
141
126
109
70
60
44
32
19
15
11
11
10
5
5
4
4
2
2
1
1
1
1
1
Options
Immunogen Human granulocytes
Clone MEM-174
Isotype IgG2a
Specificity The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
HLDA VI, WS Code BP 310
HLDA VI, WS Code M 18
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Integrin alpha M (ITGAM) antibody (FITC) (ABIN93959) Surface staining of human peripheral blood with anti-human CD11b (MEM-174) FITC.
Product cited in: Hasan, Osickova, Bumba, Novák, Sebo, Osicka: "Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding." in: FEBS letters, Vol. 589, Issue 3, pp. 374-9, 2015 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).