CD4 Molecule (CD4) antibody (FITC)

Details for Product No. ABIN118485
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Clonality (Clone)
Monoclonal ()
Flow Cytometry (FACS)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN118485
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Immunogen Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Clone RPA-T4
Isotype IgG1
Specificity This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
Purification Affinity chromatography on Protein G.
Alternative Name CD4
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
NCBI Accession NP_000607
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL of lysed whole peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-CD4 Molecule (CD4) antibody (FITC) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4:FITC
Background publications Voehringer, Koschella, Pircher: "Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1)." in: Blood, Vol. 100, Issue 10, pp. 3698-702, 2002 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe et al.: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

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