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CD4 antibody (CD4) AA 385-457 Primary Antibody

CD4 Reactivity: Human, Mouse, Rat, Chicken, Pig ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (4 references) Independent Validation (1 validation)
Catalog No. ABIN671376
$374.00
Plus shipping costs $45.00
100 μL
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  • Target See all CD4 Antibodies
    CD4
    Binding Specificity
    • 25
    • 16
    • 16
    • 16
    • 16
    • 15
    • 15
    • 9
    • 8
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 385-457
    Reactivity
    • 411
    • 241
    • 132
    • 51
    • 24
    • 24
    • 21
    • 20
    • 19
    • 17
    • 17
    • 16
    • 11
    • 11
    • 11
    • 10
    • 10
    • 9
    • 8
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Pig
    Host
    • 447
    • 148
    • 141
    • 4
    • 2
    • 2
    • 1
    Rabbit
    Clonality
    • 602
    • 134
    • 4
    Polyclonal
    Conjugate
    • 251
    • 78
    • 65
    • 59
    • 38
    • 16
    • 13
    • 13
    • 12
    • 11
    • 10
    • 9
    • 9
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD4 antibody is un-conjugated
    Application
    • 605
    • 153
    • 144
    • 132
    • 109
    • 99
    • 96
    • 41
    • 39
    • 38
    • 37
    • 35
    • 9
    • 9
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Predicted Reactivity
    Dog,Cow,Sheep,Guinea Pig
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029634 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Confocal Imaging Core, Beth Israel Deaconess Medical Center
    No.
    #029634
    Date
    03/26/2014
    Antigen
    Lot Number
    130703
    Method validated
    Immunohistochemistry
    Positive Control
    Mouse spleen tissue
    Negative Control
    Mouse muscle tissue
    Notes
    Signal was detected in positive control sample and not in negative control sample.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: CD4 Molecule (CD4)
    • Catalog number: ABIN671376
    • Lot number: 130703
    Secondary Antibody
    • Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)
    Full Protocol
    • Immunohistochemistry was performed manually.
    • Sections were deparaffinized and rehydrated.
    • Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
    • Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
    • Sections were washed x 3 in Tris buffered saline (TBS).
    • Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
    • Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
    • Sections were washed x 3 in Tris buffered saline.
    • Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
    • Sections were washed x 3 in Tris buffered saline.
    • Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
    • Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
    • Sections were washed x 3 in Distilled Water.
    • Sections were counterstained with hematoxylin for 20 sec.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.
    Experimental Notes
    - No experimental challenges noted.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Jaime-Ramirez, Yu, Caserta, Yoo, Zhang, Lee, Hofmeister, Lee, Kumar, Pan, Kumar, Baiocchi, Teknos, Pichiorri, Kaur, Old: "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." in: Molecular therapy oncolytics, Vol. 5, pp. 87-96, (2017) (PubMed).

    Zhang, Fu, Li, Wang, Zhang, Liu: "Bone morphogenetic protein 7 is associated with the nodal invasion of colon cancer." in: Oncology letters, Vol. 11, Issue 3, pp. 1707-1712, (2016) (PubMed).

    Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, (2015) (PubMed).

  • Target
    CD4
    Alternative Name
    CD4 (CD4 Products)
    Synonyms
    cd4, L3T4, Ly-4, CD4mut, W3/25, p55, CD4 molecule, carcinoembryonic antigen-related cell adhesion molecule 5, T-cell surface glycoprotein CD4, CD4 antigen, Cd4 molecule, CD4, LOC100125525, cd4, Cd4
    Background

    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

    Subcellular location: Cell membrane

    Synonyms: T-cell surface glycoprotein CD4, Cd4, T-cell surface antigen T4/Leu-3, W3/25 antigen

    Gene ID
    24932
    UniProt
    P05540
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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