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Details for Product No. ABIN120466

Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (PE)

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Antigen
Synonyms B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, Lca, RT7, Cd45, Ly-5, Lyt-4, loc, cd45, PTPRC, si:dkeyp-47c5.1
Reactivity
»Alternatives Rat (Rattus)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 6 references available
Catalog no. ABIN120466
Quantity 100 tests
Price
489.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Rat thymocyte membrane glycoproteins.
Clone OX-1
Isotype IgG1
Specificity This antibody recognises CD45. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.
Alternative Name CD45 / LCA
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Alternate names: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 24699
NCBI Accession NP_001103357.1
UniProt P04157
Application Notes Flow cytometry: use 10 ul of neat antibody to label 10e6 cells in 100 ul. (This product is routinely tested in flow cytometry on rat splenocytes). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL distilled water.
Buffer PBS, pH 7.4, containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Preservative Sodium azide
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Product cited in: Eipel, Bordel, Nickels et al.: "Impact of leukocytes and platelets in mediating hepatocyte apoptosis in a rat model of systemic endotoxemia." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 286, Issue 5, pp. G769-76, 2004 (PubMed).

Background publications Sunderland, McMaster, Williams: "Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes." in: European journal of immunology, Vol. 9, Issue 2, pp. 155-9, 1979 (PubMed).

Woollett, Barclay, Puklavec et al.: "Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes." in: European journal of immunology, Vol. 15, Issue 2, pp. 168-73, 1985 (PubMed).

Martín, Molina, Bricio et al.: "Passive dual immunization against tumour necrosis factor-alpha (TNF-alpha) and IL-1 beta maximally ameliorates acute aminonucleoside nephrosis." in: Clinical and experimental immunology, Vol. 99, Issue 2, pp. 283-8, 1995 (PubMed).

Murakami, Sato, Nagasawa et al.: "Regulation of mast cell signaling through high-affinity IgE receptor by CD45 protein tyrosine phosphatase." in: International immunology, Vol. 12, Issue 2, pp. 169-76, 2000 (PubMed).

Sato, Balla, Otterbein et al.: "Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 6, pp. 4185-94, 2001 (PubMed).

Alternatives for antigen "Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)", type "Antibodies"
Hosts (754), (464), (122), (2), (1)
Reactivities (681), (483), (145), (58), (46), (41), (37), (24), (22), (12), (7), (5), (5), (3), (2), (2), (1), (1), (1)
Applications (1077), (269), (263), (195), (191), (117), (91), (85), (67), (56), (40), (14), (10), (7), (4), (2), (2), (2), (1), (1), (1), (1)
Conjugates (197), (135), (130), (65), (26), (21), (11), (10), (9), (9), (9), (9), (8), (7), (7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (10), (6), (5), (5), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
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