CD45 antibody (C-Term)

Catalog No. ABIN6254210

The Goat Polyclonal anti-CD45 antibody is suitable to detect CD45 in samples from Human, Mouse, Rat, Dog, Chicken, Cow, Cat, Rabbit, Sheep, Guinea Pig, Pig, Goat, Monkey, Horse, Hamster, Donkey and Avian. It has been validated for WB, IHC (p) and IHC (fro).

Quick Overview for CD45 antibody (C-Term) (ABIN6254210)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Cow, Cat, Rabbit, Sheep, Guinea Pig, Pig, Goat, Monkey, Horse, Hamster, Donkey, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD45 Antibody

Binding Specificity: C-Term

Purpose: Anti-CD45

Sequence: ANCVNPLGAP EKLPEAKEQA EGSEPTSGTE GPEHSVNGPA SPALNQGS

Specificity: Using Jurkat cell lysate detects a band 180-220 kDa by Western blot.

Characteristics: Protein tyrosine phosphatase, receptor type, C

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 1,260 aa to the C-terminus of human CD45 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Background:

Goat polyclonal antibody to CD45. It is a 180-220 kDa type I transmembrane glycoprotein and a member of the protein tyrosine phosphatase (PTP) family. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It is expressed in all hematopoietic cells, but highest on lymphocytes. PTPs are known to be signalling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. It has intrinsic tyrosine phosphatase activity and it is essential for T and B cell antigen receptor-mediated activation. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signalling. CD45 also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signalling. The cross-linking of CD45 induces apoptosis in lymphocytes.

B220, CD45 antigen, CD45R, GP180, L-CA, LCA, leukocyte common antigen, LY5, protein tyrosine phosphatase, PTPRC, receptor type c polypeptide, receptor-type tyrosine-protein phosphatase C, RP11-553K8.4, T200, T200 glycoprotein, T200 leukocyte common antigen antibody.

Molecular Weight: 180-220 kDa,220 kDa

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling