Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody

Catalog No. ABIN120648

Quick Overview for Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody (ABIN120648)

Target: Ig

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: MEM-09

Isotype: IgG1

Product Details

Binding Specificity: Chain kappa, Light Chain

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity Chromatography on Protein A

Immunogen: Crude thymus membrane fraction

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Western Blotting. Immunohistochemistry (paraffin sections). Immunocytochemistry. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Ig

Alternative Name: Immunoglobulin

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.