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Details for Product No. ABIN120652

phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (AA 97-432) antibody

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Antigen
Synonyms CBP, PAG, Cbp, Pag, F730007C19Rik, MGC80192
Epitope
»Alternatives AA 97-432
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 1 reference available
Catalog no. ABIN120652
Quantity 0.1 mg
Price
346.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
Clone MEM-255
Isotype IgG2a
Purification Protein-A affinity chromatography
Purity > 95% pure (by SDS-PAGE)
Alternative Name PAG1 / Csk-binding protein
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
UniProt Q9NWQ8
Application Notes Flow Cytometry: 2 mg/ml (intracellular staining, permeabilization required). Western Blotting: Csk binding protein is an ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane microdomains (rafts), which however migrates on SDS- PAGE gels anomalously as an 80 kDa molecule. Immunohistochemistry (paraffin sections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
Brdickova, Brdicka, Andera et al.: "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton." in: FEBS letters, Vol. 507, Issue 2, pp. 133-6, 2001 (PubMed).

Alternatives for antigen "phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1)", type "Antibodies"
Hosts (35), (27)
Reactivities (59), (23), (19), (6)
Applications (34), (27), (26), (13), (10), (4)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (16), (9), (1)
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