PAG1 antibody (phosphoprotein Associated with Glycosphingolipid Microdomains 1) (C-Term)

Details for Product anti-PAG1 Antibody No. ABIN94270
Antigen
Epitope
C-Term
19
13
4
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)
68
41
34
15
2
2
1
1
1
Host
Mouse
51
21
Clonality (Clone)
Monoclonal ()
Conjugate
This PAG1 antibody is un-conjugated
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
41
26
20
13
13
9
7
6
1
Options
Immunogen C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.
Clone PAG-C1
Isotype IgG2b
Specificity The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Cross-Reactivity (Details) Mouse, Human, Rat, Bovine, Other not tested
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name PAG / Cbp (PAG1 Antibody Abstract)
Background PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Pathways p53 Signaling, TCR Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma c...
Image no. 2 for anti-phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) (C-Term) antibody (ABIN94270) Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of ly...
Product cited in: Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 (PubMed).

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 (PubMed).