Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-244) antibody

Details for Product No. ABIN120727
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Antigen
Synonyms BG:DS00004.9, CG1264, Dm lab, DmLab, Dmel\\CG1264, Dmlab, EfR9, F121, F24, F90, F90-2, Lab, l(3)01241, l(3)84Ac, lb, LAB, NTAL, WBSCR15, WBSCR5, WSCR5, Ntal, Wbscr5, AW125574, Wbscr15, LAT2, MGC139435
Epitope
AA 91-244
(21), (10), (8), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(63), (12), (5), (1)
Host
Mouse
(47), (28), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(56), (22), (22), (17), (11), (10), (9), (7), (3), (3), (1)
Pubmed 1 reference available
Catalog no. ABIN120727
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
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Immunogen Recombinant cytoplasmic domain (aa 91-244) of human NTAL
Clone NAP-07
Isotype IgG1
Purification Protein A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Alternative Name LAT2 / WBSCR15
Background NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
UniProt Q9GZY6
Application Notes Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/ml, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date 12 months
General Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Hosts (47), (28), (2)
Reactivities (63), (12), (5), (1)
Applications (56), (22), (22), (17), (11), (10), (9), (7), (3), (3), (1)
Conjugates (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (21), (10), (8), (7), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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