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LAT2 antibody (Linker For Activation of T Cells Family, Member 2) (AA 91-244)

Details for Product anti-LAT2 Antibody No. ABIN120727, Supplier: Log in to see
Antigen
  • BG:DS00004.9
  • CG1264
  • Dm lab
  • DmLab
  • Dmel\\CG1264
  • Dmlab
  • EfR9
  • F121
  • F24
  • F90
  • F90-2
  • Lab
  • l(3)01241
  • l(3)84Ac
  • lb
  • LAT2
  • MGC139435
  • LAB
  • NTAL
  • WBSCR15
  • WBSCR5
  • WSCR5
  • AW125574
  • Wbscr15
  • Wbscr5
  • Ntal
Alternatives
anti-Human LAT2 antibody for Immunocytochemistry
Epitope
AA 91-244
16
16
16
13
11
10
9
6
5
5
5
4
3
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
92
21
15
3
2
Host
Mouse
79
26
10
Clonality (Clone)
Monoclonal ()
Conjugate
This LAT2 antibody is un-conjugated
7
6
5
3
3
3
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
101
42
22
17
16
11
7
5
3
2
2
1
Supplier
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Immunogen Recombinant cytoplasmic domain (aa 91-244) of human NTAL
Clone NAP-07
Isotype IgG1
Specificity The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Purification Protein A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Alternative Name LAT2 / WBSCR15 (LAT2 Antibody Abstract)
Background NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
Gene ID 7462
UniProt Q9GZY6
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.
Expiry Date 12 months
Background publications Brdicka, Imrich, Angelisová et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).