LAT2 antibody (Linker For Activation of T Cells Family, Member 2) (AA 91-243) (PE)

Details for Product anti-LAT2 Antibody No. ABIN94440
Antigen
Epitope
AA 91-243, Cytoplasmic Domain
15
13
10
10
10
9
8
8
8
5
4
4
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
109
39
27
3
3
2
1
1
1
1
1
Host
Mouse
97
28
7
Clonality (Clone)
Monoclonal ()
Conjugate
This LAT2 antibody is conjugated to PE
8
6
4
3
3
3
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
117
39
28
26
14
14
13
3
3
3
2
1
1
Options
Immunogen Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Clone NAP-07
Isotype IgG1
Specificity The antibody NAP-07 reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Cross-Reactivity (Details) Mouse, Human, Other not tested
Antigen
Alternative Name NTAL / LAB (LAT2 Antibody Abstract)
Background NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes The reagent is designed for Flow Cytometry analysis.Suggested working dilution is 1:30. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (PE) (ABIN94440) Confocal microscopy Subcellular localization of NTAL by confocal microscopy in THP-1 ...
Product cited in: Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, 2004 (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004 (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).