LAT2 antibody (AA 196-212)

Catalog No. ABIN94442

Product Details anti-LAT2 Antibody

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Binding Specificity: AA 196-212

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody NAP-03 recognizes a defined intracellular epitope (aa 196-212) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B cells, NK cells and myeloid cells.

No Cross-Reactivity: Mouse

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Peptide corresponding to aa 196-212 of human NTAL coupled to KLH.

Clone: NAP-03

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL, positive tissue: tonsil, germinal center.
Western blotting: Recommended dilution: 1-2 μg/mL, incubation 60 min, positive control: RAMOS human lymphoma cell line, non-reducing conditions, 12 % separating SDS-PAGE gel.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-LAT2 antibody (ABIN94442)

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Target Details for LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Alternative Name: NTAL / LAB (LAT2 Products)

Background: Linker for activation of T cells family member 2,NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.,LAB, LAT2

Gene ID: 7462

UniProt: Q9GZY6

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling