Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)
Specificity
The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.
Cross-Reactivity (Details)
Mouse, Human, Other not tested
Purification
Purified by protein-A affinity chromatography.
Purity
> 95 % (by SDS-PAGE)
Immunogen
Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
Verified
LAT2
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
Western blotting: Recommended dilution: 1-2 μg/mL. Flow cytometry: Intracellular staining, recommended dilution: 1-4 μg/mL, positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Do not freeze.
Storage
4 °C
Storage Comment
Store at 2-8°C. Do not freeze.
Duque-Afonso, Yalcin, Berg, Abdelkarim, Heidenreich, Lübbert: "The HDAC class I-specific inhibitor entinostat (MS-275) effectively relieves epigenetic silencing of the LAT2 gene mediated by AML1/ETO." in: Oncogene, Vol. 30, Issue 27, pp. 3062-72, (2011) (PubMed).
Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, (2007) (PubMed).
Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).
Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).
Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).
Target
LAT2
(Linker For Activation of T Cells Family, Member 2 (LAT2))
LAB antibody, NTAL antibody, WBSCR15 antibody, WBSCR5 antibody, WSCR5 antibody, LAT2 antibody, MGC139435 antibody, Ntal antibody, Wbscr5 antibody, AW125574 antibody, Wbscr15 antibody, linker for activation of T-cells family member 2 antibody, linker for activation of T cells family, member 2 antibody, LAT2 antibody, Lat2 antibody
Background
Linker for activation of T cells family member 2,NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.,LAB, LAT2