Histone H4 antibody (N-Term)

Catalog No. ABIN127256

The Rabbit Polyclonal anti-Histone H4 antibody has been validated for WB, IP and IHC (p). It is suitable to detect Histone H4 in samples from Human, Mouse and Rat.

Quick Overview for Histone H4 antibody (N-Term) (ABIN127256)

Target: Histone H4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Histone H4 Antibody

Binding Specificity: N-Term

Specificity: The antibody detects ~11 kDa Histone H4 protein. It does not cross-react with other Histones.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to the N-terminus of Human Histone H4.

Application Details

Application Notes: Western Blot: 0.5-4.0 μg/mL. Immunoprecipitation: 20-40 μg/mL. Immunohistochemistry: 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Histone H4

Target: Histone H4

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.Synonyms: H4/A, H4FA, HIST1H4

Gene ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805