CD8 alpha antibody (FITC)

Catalog No. ABIN135119

The Rat Monoclonal anti-CD8 alpha antibody has been validated for FACS. It is suitable to detect CD8 alpha in samples from Mouse. There are 2+ publications available.

Quick Overview for CD8 alpha antibody (FITC) (ABIN135119)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 53-6-7

Product Details anti-CD8 alpha Antibody (FITC)

Specificity: Mouse CD8α, Mr 32-34 kDa

Characteristics: Rat Anti-Mouse CD8a-FITC

Immunogen: Spleen cells or thymocyte membranes

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Depletion - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 2 g/106 cells FITC and BIOT conjugates 2 g/106 cells AF488 conjugate 1 g/106 cells PE, APC, SPRD, PE/TXRD, CY5, PE/CY5.5, PE/CY7, 0.2 g/106 cells APC/CY5.5, APC/CY7, PACBLU, AF647, and AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vivo and in vitro depletion of CD8a+ cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg or 0.1 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 alpha antibody (FITC) (ABIN135119)

Hintermann, Ehser, Christen: "The CYP2D6 Animal Model: How to Induce Autoimmune Hepatitis in Mice." in: Journal of visualized experiments : JoVE, Issue 60, (2012) (PubMed).

Safari, Dekker, Rijkers, Snippe: "Codelivery of adjuvants at the primary immunization site is essential for evoking a robust immune response to neoglycoconjugates." in: Vaccine, (2010) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background: In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells.

Pathways: TCR Signaling