ITGA6 antibody (PE)

Catalog No. ABIN181782

Product Details anti-ITGA6 Antibody (PE)

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Reactivity: Human, Mouse, Dog, Pig, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA6 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD49F (VLA-6 alpha chain). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts.VLA-6 is an important mediator of cell binding to laminin. Studies suggest that clone GoH3 specifically blocks the binding of cells to the E8 fragment of laminin. We recommend the use of SM038Afor this purpose.

Purification: Affinity chromatography on Protein G

Immunogen: BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.

Clone: NKI-GoH3

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Neat - 1/5, Use 10 μL of the suggested working dilution to label 10^6 platelets in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA6

Target: ITGA6 (Integrin, alpha 6 (ITGA6))

Alternative Name: CD49f / ITGA6 (ITGA6 Products)

Background: CD49F (VLA-6 alpha chain)is a 120kD cell surface glycoprotein that can form distinct complexes with CD29 (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Clone NKI-GoH3 reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1).Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6

Gene ID: 3655

NCBI Accession: NP_000201

UniProt: P23229

Pathways: CXCR4-mediated Signaling Events, Brown Fat Cell Differentiation, Integrin Complex