CD8B antibody (PE)

Catalog No. ABIN181906

Product Details anti-CD8B Antibody (PE)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Chicken, Avian

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the alpha chain of the CD8 homologue, a 33-35 kDa cell surface protein. Clone 11-39 recognises all polymorphic forms of chicken CD8 alpha.

Purification: Affinity chromatography on Protein G

Immunogen: Chicken T-cells. Lymph node cells from immunised Balb/c mice were fused with cells of the SP2/0 myeloma cell line.

Clone: 01-11-39

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: Use of Sodium Azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the lyophilized antibody at 2-8 °C. Store the antibody after reconstitution for several weeks at 2-8 °C. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at -20 °C. The concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8 beta (CD8B Products)

Background: CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells.Synonyms: CD8B, CD8B1, T-cell surface glycoprotein CD8 beta chain

Gene ID: 396175

NCBI Accession: NP_990578

UniProt: Q90769

Pathways: TCR Signaling