CD8B antibody (PE)

Catalog No. ABIN7013938

The Rat Monoclonal anti-CD8B antibody is suitable to detect CD8B in samples from Mouse. It has been validated for FACS.

Quick Overview for CD8B antibody (PE) (ABIN7013938)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8B antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: H35-17-2

Product Details anti-CD8B Antibody (PE)

Purpose: Anti-Ms CD8b PE

Characteristics: The rat monoclonal antibody H35-17.2 recognizes an extracellular epitope of mouse CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on cytotoxic T cells and thymocytes.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Mouse CD8b

Isotype: IgG2b, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b

Background: The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.,Ly-3, Ly-C, Lyt-3

Gene ID: 35

UniProt: P10300

Pathways: TCR Signaling