PSMD4/ASF antibody (C-Term)

Catalog No. ABIN182537

The Rabbit Polyclonal anti-PSMD4/ASF antibody is suitable to detect PSMD4/ASF in samples from Human, Rat, Mouse, Cow, Sheep, Rabbit, Dog, Guinea Pig, Horse and Pig. It has been validated for WB and IHC.

Quick Overview for PSMD4/ASF antibody (C-Term) (ABIN182537)

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Reactivity: Human, Rat, Mouse, Cow, Sheep, Rabbit, Dog, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD4/ASF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PSMD4/ASF Antibody

Binding Specificity: C-Term

Purpose: PSMD4 Antibody - C-terminal region

Sequence: VMQDPEFLQS VLENLPGVDP NNEAIRNAMG SLASQATKDG KKDKKEEDKK

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against PSMD4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PSMD4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32341-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for PSMD4/ASF

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Alternative Name: PSMD4

Background:

Targeted Subunit: 4

Background Information: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMD4 encodes one of the non-ATPase subunits of the 19S regulator lid.

Gene Name: Proteasome (prosome, macropain) 26S subunit, non-ATPase, 4

Alternative Symbols: AF, ASF, S5A, AF-1, MCB1, Rpn10, pUB-R5

Protein Name: 26S proteasome non-ATPase regulatory subunit 4

Molecular Weight: 41kDa

Gene ID: 5710

NCBI Accession: NP_002801

UniProt: P55036

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway