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Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody

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Antigen
Alternatives
Epitope
Middle Region
(12), (8), (4), (3), (3), (3), (2), (1), (1), (1)
Reactivity
Chicken, Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(66), (32), (32), (6), (6), (4), (2), (1), (1)
Host
Rabbit
(49), (9), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(55), (27), (14), (14), (11), (10), (2), (2), (1), (1)
Pubmed 2 references available
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Quantity 100 μg
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Immunogen Synthetic peptide directed towards the middle region of human UBE2N
Sequence VDKLGRICLD ILKDKWSPAL QIRTVLLSIQ ALLSAPNPDD PLANDVAEQW
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Sheep : 100 %, Zebrafish : 100 %, Dog : 80 %
Characteristics This is a rabbit polyclonal antibody against UBE2N. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name UBE2N (UBE2N Antibody Abstract)
Background UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Molecular Weight 17 kDa
Gene ID 7334
NCBI Accession NP_003339, NM_003348
UniProt P61088
Pathways Fc-epsilon Receptor Signaling Pathway
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 152 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN182924) WB Suggested Anti-UBE2N Antibody Titration: 1.25ug/ml
Positive Control: ...
Western Blotting (WB) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN182924) WB Suggested Anti-UBE2N
Antibody Titration: 1.25 µg/mL
Positive Control...
Immunohistochemistry (IHC) image for anti-Ubiquitin-Conjugating Enzyme E2N (UBE2N) (Middle Region) antibody (ABIN182924) Sample Type: Human brain stem cells

Primary Antibody Dilution: 1:500
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Product cited in: Xu, Yan, Jovanovic et al.: "Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts." in: Proteomics, Vol. 10, Issue 13, pp. 2458-70, 2010 (PubMed).

Background publications Bothos, Summers, Venere et al.: "The Chfr mitotic checkpoint protein functions with Ubc13-Mms2 to form Lys63-linked polyubiquitin chains." in: Oncogene, Vol. 22, Issue 46, pp. 7101-7, 2003 (PubMed).

Catalog No. ABIN182924

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