UBE2N antibody (C-Term)

Catalog No. ABIN324309

Product Details anti-UBE2N Antibody

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Sheep, Drosophila melanogaster, Chicken, Xenopus laevis, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human UBE2N

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human UBE2N (P61088, NP_003339). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Xenopus, Trout, Catfish, Salmon, Pike, Zebrafish, Drosophila, Slime mold, Beetle, Arabidopsis (100%), Sea squirt (92%), Rice (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBE2N / UBC13 (UBE2N Products)

Background: Name/Gene ID: UBE2N

Synonyms: UBE2N, BLU, UbcH13, UbcH-ben, Ubiquitin carrier protein N, Yeast UBC13 homolog, UBC13, Ubiquitin-protein ligase N

Gene ID: 7334

NCBI Accession: NP_003339

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway