UBE2N antibody (Middle Region)

Catalog No. ABIN2778258

Product Details anti-UBE2N Antibody

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Dog, Cow, Guinea Pig, Horse, Rabbit, Sheep, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VDKLGRICLD ILKDKWSPAL QIRTVLLSIQ ALLSAPNPDD PLANDVAEQW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2N. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2N

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 152 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE2N antibody (ABIN2778258)

Xu, Yan, Jovanovic, An, Cheng, Aakre, Yi, Eng, Link, Moses: "Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts." in: Proteomics, Vol. 10, Issue 13, pp. 2458-70, (2010) (PubMed).

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBE2N (UBE2N Products)

Background: UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Alias Symbols: UBC13, UbcH-ben
Protein Interaction Partner: TRIM33, BFAR, MALT1, STUB1, BCL10, UBE2V2, UBC, TRAF6, ZNRF1, XIAP, SLX8, SLX5, MARCH7, TNF, UBE2N, RNF168, RNF8, PARK2, OTUB1, ARIH1, NEDD4L, MFN2, TRIM72, PELI3, RNF111, RNF11, BAG3, TRAF2, FN1, VCAM1, RNF126, RNF115, NBN, ATP6V1H, VPS33B, ZDHHC5, ACTR2
Protein Size: 152

Molecular Weight: 17 kDa

Gene ID: 7334

NCBI Accession: NM_003348, NP_003339

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway