Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody

Details for Product No. ABIN183598
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Antigen
Synonyms Idb4, bHLHb27, cb547, fb77d04, sb:cb547, wu:fb77d04, zgc:123214, bhlhb27, id4, id4a, idb4, IDB4
Epitope
N-Term
(16), (10), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Xenopus laevis, Human, Chicken
(47), (29), (26), (14), (13), (1), (1), (1), (1)
Host
Rabbit
(41), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(36), (24), (10), (10), (9), (6), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN183598
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Immunogen Synthetic peptide directed towards the N terminal of human ID4
Sequence MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSAA AAAAAAAARC
Predicted Reactivity Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, African clawed frog : 80 %, Chicken : 70 %
Characteristics This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name ID4
Background Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).
Molecular Weight 17 kDa
Gene ID 3400
NCBI Accession NP_001537, NM_001546
UniProt P47928
Research Area Stem Cells, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 161 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody WB Suggested Anti-ID4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1562500
Positive Control: HepG2 cell lysate
anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody (2) Host: Rabbit
Target Name: ID4
Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 2.5 µg/mL Peptide Concentration:..0 µg/mL Lysate Quantity:.41 µg/laneGel Concentration: 12 %
Background publications Yu, Liu, Vandeusen et al.: "Global assessment of promoter methylation in a mouse model of cancer identifies ID4 as a putative tumor-suppressor gene in human leukemia." in: Nature genetics, Vol. 37, Issue 3, pp. 265-74, 2005 (PubMed).

Validation Images
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