You are viewing an incomplete version of our website. Please click to reload the website as full version.

Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody

Details for Product No. ABIN183598, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

(25), (18), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Chicken, Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(78), (36), (31), (4), (3), (2), (2), (1), (1)
(59), (19)
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(55), (35), (21), (14), (11), (9), (7)
Pubmed 2 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 100 μg
Shipping to United States ( )
Immunogen Synthetic peptide directed towards the N terminal of human ID4
Predicted Reactivity Dog : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, African clawed frog : 80 %, Chicken : 70 %
Characteristics This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name ID4 (ID4 Antibody Abstract)
Background Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).
Molecular Weight 17 kDa
Gene ID 3400
NCBI Accession NP_001537, NM_001546
UniProt P47928
Research Area Stem Cells, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 161 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody (ABIN183598) WB Suggested Anti-ID4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1...
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody (ABIN183598) Host: Rabbit
Target Name: ID4
Sample Tissue: HepG2Lane A: Primary Anti...
Product cited in: Melcher, Hartmann, Zopf et al.: "LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability." in: Carcinogenesis, Vol. 32, Issue 4, pp. 636-42, 2011 (PubMed).

Background publications Yu, Liu, Vandeusen et al.: "Global assessment of promoter methylation in a mouse model of cancer identifies ID4 as a putative tumor-suppressor gene in human leukemia." in: Nature genetics, Vol. 37, Issue 3, pp. 265-74, 2005 (PubMed).

Catalog No. ABIN183598

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)