ID4 antibody (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein) (N-Term)

Details for Product anti-ID4 Antibody No. ABIN2780374, Supplier: Log in to see
Antigen
  • Idb4
  • bHLHb27
  • cb547
  • fb77d04
  • sb:cb547
  • wu:fb77d04
  • zgc:123214
  • bhlhb27
  • id4
  • id4a
  • idb4
  • IDB4
  • inhibitor of DNA binding 4
  • inhibitor of DNA binding 4, HLH protein
  • inhibitor of DNA binding 4, HLH protein S homeolog
  • Id4
  • id4
  • id4.S
  • ID4
Epitope
N-Term
18
17
5
3
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish
78
50
37
5
4
2
2
1
1
1
1
1
Host
Rabbit
58
20
Clonality
Polyclonal
Conjugate
This ID4 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
55
32
22
15
13
10
7
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ID4
Sequence MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC
Predicted Reactivity Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name ID4 (ID4 Antibody Abstract)
Background Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).[supplied by OMIM].
Alias Symbols: IDB4, bHLHb27
Protein Interaction Partner: TCF3, TCF4, ID3, MYOD1, HES1,
Protein Size: 161
Molecular Weight 17 kDa
Gene ID 3400
NCBI Accession NM_001546, NP_001537
UniProt P47928
Research Area Stem Cells, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 161 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody (ABIN2780374) WB Suggested Anti-ID4 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:1...
Western Blotting (WB) image for anti-Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4) (N-Term) antibody (ABIN2780374) Host: Rabbit
Target Name: ID4
Sample Tissue: HepG2Lane A: Primary Anti...
Product cited in: Melcher, Hartmann, Zopf, Herterich, Wilke, Müller, Rosler, Kudlich, Al-Taie, Rosenwald, Katzenberger, Scholtka, Seibold, Rogoll, Scheppach, Scheurlen, Lührs: "LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability." in: Carcinogenesis, Vol. 32, Issue 4, pp. 636-42, 2011 (PubMed).

Background publications Yu, Liu, Vandeusen, Becknell, Dai, Wu, Raval, Liu, Ding, Mao, Liu, Smith, Lee, Rassenti, Marcucci, Byrd, Caligiuri, Plass: "Global assessment of promoter methylation in a mouse model of cancer identifies ID4 as a putative tumor-suppressor gene in human leukemia." in: Nature genetics, Vol. 37, Issue 3, pp. 265-74, 2005 (PubMed).

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