ID4 antibody (N-Term)

Catalog No. ABIN952816

This Rabbit Polyclonal antibody specifically detects ID4 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for ID4 antibody (N-Term) (ABIN952816)

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ID4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ID4 Antibody

Binding Specificity: AA 1-30, N-Term

Specificity: This antibody recognizes Human and Mouse ID4 / BHLHB27 (N-term).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human ID4 / BHLHB27

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ID4

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Alternative Name: ID4 / BHLHB27

Background: ID4 is transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins.Synonyms: Class B basic helix-loop-helix protein 27, DNA-binding protein inhibitor ID-4, Inhibitor of DNA binding 4

Molecular Weight: 16622 Da

Gene ID: 3400

NCBI Accession: NP_001537