Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody

Details for Product No. ABIN185371
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, AI415265, AI747682, Narc1
Epitope
C-Term
(9), (7), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(53), (23), (21)
Host
Goat
(42), (14), (2), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(47), (30), (17), (12), (11), (10), (6), (5), (2), (2), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN185371
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Available

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence CRSRHLAQASQELQ, from the C Terminus of the protein sequence according to NP_777596.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PCSK9
Background Synonyms: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin/kexin type 9, LDLCQ1, NARC-1
Gene ID 255738
NCBI Accession NP_777596.2
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75 kDa band observed in Human Adipose and Pancreas lysates (calculated MW of 74.3 kDa according to NP_777596.2). Recommended concentration: 0.3-1 µg/mL. Detects transfected human pcsk9 in McA cell lysates (see figure) as two bands of approx. 73 and 60 kDa, representing unprocessed and processed protein. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1,14(9):1161-9, PMID: 15772090. Data obtained from antibodies from a previous goat. An anonymous customer found positive results in WB on Human Liver.
IHC: In paraffin embedded Human Kidney shows ER and nuclear membrane staining in PCT. Recommended concentration: 2-6 µg/mL. Data obtained from antibodies from a previous goat.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody ABIN185371 staining (0.2 µg/mL) of McA cell lysates: untransfected (1), transfected with wild type human pcsk9 (2), transfected with S127R human pcsk9 (3). Data kindly provided by Dr. X-M Sun, Hammersmith Hospital, London.
anti-Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody (2) ABIN185371 (0.3 µg/mL) staining of Human Adipose lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody (3) ABIN185371 (4 µg/mL) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
General Cohen, Pertsemlidis, Kotowski et al.: "Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9." in: Nature genetics, Vol. 37, Issue 2, pp. 161-5, 2005 (PubMed).

Lalanne, Lambert, Amar et al.: "Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1312-9, 2005 (PubMed).

Hosts (42), (14), (2), (2)
Reactivities (53), (23), (21)
Applications (47), (30), (17), (12), (11), (10), (6), (5), (2), (2), (1), (1), (1)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (7), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top