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Details for Product No. ABIN185371

Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) (C-Term) antibody

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Synonyms FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, AI415265, AI747682, Narc1
»Alternatives C-Term
»Alternatives Human
Clonality Polyclonal
»Alternatives Un-conjugated
Methode Type Peptide ELISA
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN185371
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence CRSRHLAQASQELQ, from the C Terminus of the protein sequence according to NP_777596.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PCSK9
Background Synonyms: PCSK9, FH3, NARC1, HCHOLA3, proprotein convertase subtilisin/kexin type 9, neural apoptosis regulated convertase 1, hypercholesterolemia, autosomal dominant 3, proprotein convertase subtilisin/kexin type 9, LDLCQ1, NARC-1
Gene ID 255738
NCBI Accession NP_777596.2
Research Area Proteolysis / Ubiquitin, Metabolism, Amino Acids
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 75 kDa band observed in Human Adipose and Pancreas lysates (calculated MW of 74.3 kDa according to NP_777596.2). Recommended concentration: 0.3-1 µg/mL. Detects transfected human pcsk9 in McA cell lysates (see figure) as two bands of approx. 73 and 60 kDa, representing unprocessed and processed protein. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1,14(9):1161-9, PMID: 15772090. Data obtained from antibodies from a previous goat. An anonymous customer found positive results in WB on Human Liver.
IHC: In paraffin embedded Human Kidney shows ER and nuclear membrane staining in PCT. Recommended concentration: 2-6 µg/mL. Data obtained from antibodies from a previous goat.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Cohen, Pertsemlidis, Kotowski et al.: "Low LDL cholesterol in individuals of African descent resulting from frequent nonsense mutations in PCSK9." in: Nature genetics, Vol. 37, Issue 2, pp. 161-5, 2005 (PubMed).

Lalanne, Lambert, Amar et al.: "Wild-type PCSK9 inhibits LDL clearance but does not affect apoB-containing lipoprotein production in mouse and cultured cells." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1312-9, 2005 (PubMed).

Alternatives for antigen "Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9)", type "Antibodies"
Hosts (35), (15), (2)
Reactivities (49), (20), (18)
Applications (38), (26), (12), (10), (8), (6), (6), (2), (2), (1), (1)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (7), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)