PCSK9 antibody (AA 214-228)

Catalog No. ABIN570954

The Goat Polyclonal anti-PCSK9 antibody (ABIN570954) specifically detects PCSK9 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for PCSK9 antibody (AA 214-228) (ABIN570954)

Target: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCSK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PCSK9 Antibody

Binding Specificity: AA 214-228

Purpose: PCSK9 (aa214-228)

Sequence: TRFHRQASKC DSHGT

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TRFHRQASKCDSHGT, from the internal region of the protein sequence according to NP_777596.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70-75 kDa band observed in HepG2 cell lysates and approx 75 kDa in HeLa cell lysates (calculated MW of 74.3 kDa according to NP_777596.2).. Recommended concentration: 1-2g/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCSK9

Target: PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))

Alternative Name: PCSK9

Background: PCSK9, proprotein convertase subtilisin/kexin type 9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, OTTHUMP00000009833, convertase subtilisin/kexin type 9 preproprotein, hypercholesterolemia, autosomal dominant 3, neural apoptosis regulated convertase 1

Gene ID: 255738

NCBI Accession: NP_777596