phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Internal Region) antibody

Details for Product No. ABIN185482
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Antigen
Synonyms
PEPCK, PHOSPHOENOLPYRUVATE CARBOXYKINASE, T28I19.150, T28I19_150, phosphoenolpyruvate carboxykinase 1, 143299_at, CG10924, CG17725, Dmel\\CG17725, Dromel_CG17725_FBtr0086701_pepck_mORF, dPEPCK, pepck, ... show more
PEPCK, PHOSPHOENOLPYRUVATE CARBOXYKINASE, T28I19.150, T28I19_150, phosphoenolpyruvate carboxykinase 1, 143299_at, CG10924, CG17725, Dmel\\CG17725, Dromel_CG17725_FBtr0086701_pepck_mORF, dPEPCK, pepck, cb856, cb924, fj93h11, zgc:63869, wu:fc51c05, wu:fj93h11, pepck-c, pepck1, pepckc, PCK1, PEPCK-C, PEPCK1, PEPCKC, PPCK1, GTP, PCK, Pepck, RATPEPCK, AI265463, Pck-1, Ppc1C show less
Epitope
Internal Region
(14), (11), (7), (6), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(99), (50), (46), (30), (27), (27), (17), (1)
Host
Goat
(93), (16), (13), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(10), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(90), (58), (24), (23), (20), (9), (8), (7), (5), (3)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185482
374.27 $
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Immunogen Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PCK1 / PEPCKC
Background Synonyms: PCK1, phosphoenolpyruvate carboxykinase 1 (soluble), HGNC:8724, MGC22652, PEPCK1, PEPCKC, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, PEPCK-C
Gene ID 5105
NCBI Accession NP_002582
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave bands at approx 70 kDa, 45 kDa and 28 kDa in Human Adipose, Kidney and Liver lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in pyramidal cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Yang, Graham, Mody et al.: "Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes." in: Nature, Vol. 436, Issue 7049, pp. 356-62, 2005 (PubMed).

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