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phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1) (Internal Region) antibody

Details for Product No. ABIN185482, Supplier: Login to see
Antigen
  • PEPCK
  • PHOSPHOENOLPYRUVATE CARBOXYKINASE
  • T28I19.150
  • T28I19_150
  • phosphoenolpyruvate carboxykinase 1
  • 143299_at
  • CG10924
  • CG17725
  • Dmel\\CG17725
  • Dromel_CG17725_FBtr0086701_pepck_mORF
  • dPEPCK
  • pepck
  • cb856
  • cb924
  • fj93h11
  • zgc:63869
  • wu:fc51c05
  • wu:fj93h11
  • pepck-c
  • pepck1
  • pepckc
  • PCK1
  • PEPCK-C
  • PEPCK1
  • PEPCKC
  • PPCK1
  • GTP
  • PCK
  • Pepck
  • RATPEPCK
  • AI265463
  • Pck-1
  • Ppc1C
Epitope
Internal Region
29
19
14
12
7
6
5
4
4
3
3
2
2
2
1
1
1
1
Reactivity
Human
147
65
55
15
8
5
5
2
2
1
1
1
1
1
1
1
1
Host
Goat
123
33
16
2
Clonality
Polyclonal
Conjugate
Un-conjugated
16
11
10
4
4
2
2
2
2
2
2
2
2
2
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
126
89
49
31
20
8
8
5
4
2
Supplier
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Immunogen Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.
Blocking Peptide Blocking peptide for this product available: ABIN369218
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PCK1 / PEPCKC (PCK1 Antibody Abstract)
Background Synonyms: PCK1, phosphoenolpyruvate carboxykinase 1 (soluble), HGNC:8724, MGC22652, PEPCK1, PEPCKC, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase, PEPCK-C
Gene ID 5105
NCBI Accession NP_002582
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave bands at approx 70 kDa, 45 kDa and 28 kDa in Human Adipose, Kidney and Liver lysates after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 69.2 kDa according to NP_002582.2. All three detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in pyramidal cells. Recommended concentration: 2-4 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Yang, Graham, Mody et al.: "Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes." in: Nature, Vol. 436, Issue 7049, pp. 356-62, 2005 (PubMed).