Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (C-Term) antibody

Details for Product No. ABIN185487
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Antigen
Synonyms ENPP1, si:ch211-142e24.2, zgc:136238, ARHR2, M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, 4833416E15Rik, AI428932, C76301, CD203c, E-NPP1, Ly-41, Npps, Pca, Pca-1, Pdnp1, ttw, twy, Pc1
Epitope
C-Term
(27), (9), (8), (8), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(59), (44), (36), (28), (17)
Host
Goat
(58), (12), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(41), (27), (24), (19), (13), (11), (8), (5), (3), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN185487
374.27 $
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Immunogen Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ENPP1 / PC1
Background Synonyms: ENPP1, PC-1, ectonucleotide pyrophosphatase/phosphodiesterase 1, HGNC:3356, M6S1, NPP1, NPPS, PCA1, PDNP1, Ly-41 antigen, OTTHUMP00000043194, alkaline phosphodiesterase 1, membrane component, chromosome 6, surface marker 1, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membrane glycoprotein 1
Gene ID 5167
NCBI Accession NP_006199
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: In transfected HEK293 transiently expressing ENPP1 a band of approx. 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105 kDa according to NP_006199.2. Recommended concentration: 1-3 µg/mL.
IHC: In paraffin embedded Liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on Human Pancreas showing membranous staining of acinar cells. Recommended concentration: 1-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1) (C-Term) antibody ABIN185487 (1µg/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Product cited in: Simão, Yadav, Narisawa et al.: "Proteoliposomes harboring alkaline phosphatase and nucleotide pyrophosphatase as matrix vesicle biomimetics." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7598-609, 2010 (PubMed).

General Meyre, Bouatia-Naji, Tounian et al.: "Variants of ENPP1 are associated with childhood and adult obesity and increase the risk of glucose intolerance and type 2 diabetes." in: Nature genetics, Vol. 37, Issue 8, pp. 863-7, 2005 (PubMed).

Validation Images
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